WGBS assessment of global methylation alterations in Dnmt3aKO or Dnmt3bKO mouse embryonic stem cells. Mus musculus

Using WGBS we assessed global DNA methylation changes in Dnmt3aKO or Dnmt3bKO mouse embryonic stem cells. Compared with WT cells, Dnmt3aKO cells but not Dnmt3bKO cells showed genome-wide hypomethylation. Overall design: Genomic DNA from WT, Dnmt3aKO or Dnmt3bKO J1 ES cells wes isolated, treated with bisulfite and sequenced with Illumina HiSeq sequencing systems.