RNAseq of IL-1beta stimulated THP-1 cells

Study goals: We previously found that the dominant-active form of the inflammasome adaptor apoptosis-associated speck-like protein containing a CARD (ASC) splice variant that lacks exon2 (delta exon2) is expressed in Japanese patients with palindromic rheumatism (Suganuma et al., APJAI 2019). The study goal is to clarify the aberrant splicing mechanism of ASCs. The genomic DNA of Japanese patients with PR was sequenced. The effect of the observed SNPs on ASC splicing was determined via exon trapping using THP-1 cells stimulated with interleukin-1 beta (IL-1beta) or ceramide. To investigate the genes that affect alternative splicing via IL-1beta, we analyzed the transcriptome of IL-1beta-treated THP-1 cells using RNA sequencing.