X 染色体引物

The long-read sequencing was aligned to a reference genome using Minimap2. The parameters include: -z 600 200 -x map-ont. Telomeric sequences within these reads were then identified using Telomap (telomap wgs NA21ZD019353.bam -n DNA21ZD019353 TTAGGG -t 30 -m TTAGGG), which clusters subtelomeric regions to generate unique anchor sequences that were mapped to chromosome ends. The start positions of these telomeric sequences were converted from their positions within the long reads to their corresponding locations on the reference genome (GRCh38). These mapped telomere sequences were grouped by chromosome and read orientation. Within each chromosome and orientation group, the start and end positions of all identified telomere sequences were used to calculate the telomere length. The minimum start position and maximum end position were determined, and the difference between these values represented the estimated telomere length for that specific group. This approach allows for the accurate measurement and analysis of telomere lengths from long-read sequencing data, providing valuable insights into genome stability and cellular aging.