Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Sanger Clinical Production Pilot

The HMP clinical production pilot study represents the dideoxy sequencing of 16S rRNA genes amplified from multiple body sites across a small number of subjects. The goal of the pilot was to test the production Sanger sequencing protocol, to quantify the variation among replicates processed by various centers, and to gain a preliminary understanding of the diversity of microbial community structures among samples extracted from distinct body sites.