RNA-seq of control and Lmna -/- mice heart samples

RNA-sequencing analysis from whole heart ribosome depleted RNA from the 2-week old WT and Lmna-/- mice (N=5) . Strand specific RNA seq libraries where prepared form ribosome-depleted cardiac RNA samples using the Illumina TruSeq stranded total RNA library preparation kit. The samples weresequenced on the Illumina HiSeq 4000 instrument using the paired-end sequencing reagents to generate100 base paired end reads. Cardiac RNA profiles from 14-day old wild type (WT) and Lmna -/- mice were generated by deep sequencing from 5 mice in each genotype.本数据集针对2周龄野生型（WT）与Lmna基因敲除（Lmna-/-）小鼠的全心脏核糖体去除RNA开展RNA测序分析，每组样本量为N=5。采用Illumina TruSeq链特异性总RNA建库试剂盒，对核糖体去除的心脏RNA样本构建链特异性RNA测序文库。使用Illumina HiSeq 4000测序仪，以双端测序试剂完成测序，生成100碱基长度的双端读段。本数据集通过深度测序获取了14日龄野生型与Lmna-/-小鼠的心脏RNA表达谱，每种基因型对应5只小鼠样本。