Sequence-fitness dataset of O-methyltransferase activity for isovanillic acid production

Corresponding dataset to the following publication: https://doi.org/10.1101/2025.10.24.684421This dataset is composed of two files data_seq_to_fitness_t_0_x_no_vif.csv: each row is a unique DNA sequence OMT.csv: each row is a unique amino acid sequence Thus, OMT.csv is a processed and aggregated version of data_seq_to_fitness_t_0_x_no_vif.csv. For more details on each, see below. 1. data_seq_to_fitness_t_0_x_no_vif.csv This dataset contains 572,013 unique datapoints obtained through MillionFull for sequence-fitness relationship for the enzyme activity protocatechuic acid + SAM -> isovanillic acid + SAH. For each variant we report precision‑weighted growth‑rate estimates, promoter, coding and translated protein sequences, exhaustive mutation annotations relative to a reference parent sequence, and quality‑control metrics derived from the underlying read clusters provided by DiMSum. For more details, see 1_readme.md 2. OMT.csv First, the following variants were removed from (1): variants with a stop codon variants with frameshift mutations (indels) in the DNA coding region variants with any mutations (substitution or indel) in the promoter region variants with indels in the protein sequence Then, for every unique protein sequence, growthrates from all degenerate nucleotide sequences were averaged to obtain the column growthrate_total. If multiple nucleotide sequences were present, growthrate_sigma_total was set to the standard deviation of each growthrate_total value per nucleotide sequnce. If only one nucleotide sequence was present for the protein sequence, then the nucleotide growthrate_sigma_total was used. Columns protein seq Protein sequence growthrate_total Growthrate total growthrate_sigma_total Growthrate sigma total mutations Mutations relative to parent, in list format. For example, ['S_28_T, E_50_K'] n mutations Number of mutations Parent Boolean, True/False normalized growthrate Growthrates normalized by parent growthrate. log normalized growthrate Log of normalized growthrate. Given a floor of -1.5