m6A modification profiles in RA FLS with FTO knockdown

Fibroblast-like synoviocytes (FLS) harbour active migration, invasion, proliferation and decreased cell-contact inhibition, and served as a crucial component in pathogenesis of rheumatoid arthritis. However, the mechanism underlying the aggressive behavior remain unclear. In this study, we established FTO-knockdown RA FLS to investigate the molecular mechanism. To explore the function of m6A in regulating the aggressive behavior of RA FLS, FTO was knockdown by infected lentivirus with shRNA. meRIP-seq was performed to analyze the alteration of m6A modification and identified the potential targets.