Normalized fraction of antisense transcript signature abundance of validated miRNA targets, predicted targets and paralogous non-targets from the Massively Parallel Signature Sequencing (MPSS) Plus Databasea.

aSum of abundance for mRNA antisense signatures (classes 3 and 6, transcript per million) was collected from the MPSS Plus Database (http://mpss.udel.edu/at/, Table S2). Normalized data for all 17 libraries in MPSS database was obtained by dividing the abundance of mRNA antisense signatures for the total signatures associated with each gene, and the average for total genes in each set is presented here (percentage of mRNA antisense signatures in total signatures/locus). The abbreviation for all 17 libraries is as follows: CAF, CAS: actively growing callus; INF, INS: inflorescence; AP1: apetela1-10 mutant inflorescence; SAP: superman/apetela1 mutant inflorescence; AP3: apetela3-6 mutant inflorescence; AGM: agamous mutant inflorescence (all inflorescence samples were collected from immature buds of mixed stages); LEF, LES: untreated leaves of 21 days; S04: leaves of 4 hr after salicylic acid treatment; S52: leaves of 52 hr after salicylic acid treatment; ROF, ROS: untreated root of 21 days; SIF, SIS: silique of 24 to 48 hr post-fertilization; GSE: germinating seedlings. *: Data from the classic MPSS method; ?: data from the signature MPSS method.bn = 94.cn = 283.dn = 140.eThe difference between validated miRNA targets and paralogous non-targets for the percentage of mRNA antisense signatures in total signatures.fIndividual P-value of the Student's t-test analysis (one-sided, equal variance model) for the percentages of mRNA antisense signatures in total signatures between validated miRNA targets and paralogous non-targets in each library.gCombined P-value for the percentages of mRNA antisense signatures between validated miRNA target versus paralogous non-targets in all signature libraries (labeled by ?).