RNA-seq for splenic gene expression in response to Newcastle disease virus challenge in Hy-Line Brown chickens

In order to have a better understanding about genetic regulation of response to NDV challenge, and also to identify the candidate genes regulating disease resistance in combination with GWAS, gene expression in spleen of Hy-Line Brown chickens was investigated. The chickens were divided into two groups (8 chickens/group) that are respectively treated by NDV(200 ul of 108 EID50%) and Phosphate-buffered saline (PBS) through nasal and ocular inoculation routes at 21 days post hatch. Gene expression in spleen was then detected by RNA-seq at 2 and 6 day post inoculation (dpi).