Genetic interactions of heterochronic mutants.

1All animals examined were homozygous for null alleles of the genes indicated and carry an integrated transgene wIs78(scm::GFP; ajm-1::GFP) to mark seam cells. All alleles are null.2Seam cell counts were performed on L4 animals except where indicated.3Alae formation was assessed in the early L4 stage.4Strains carrying the let-7 mutation additionally contained a linked unc-3 mutant allele. They were grown at 15°C to limit constitutive dauer formation that results from the unc-3 mutation at higher temperatures in these backgrounds.5Seam cell fusion with no alae formation was observed in the other 85% of animals.SEM, standard error of the mean; ND, not determined.