Negative-staina EM analysis PA-msANTXR2 co-complexes following an exposure at 37°Cb.

aNegative-stain electron micrographs using uranyl acetate stain, 2%.bA pre-assembled population of PA-msANTXR2 complexes (containing 78% PA7 and 22% PA8) was incubated for 5 minutes at 37°C at the specified pH.cThe mean number of particles per micrograph (n of 10 micrographs) given as ±s.d.dOligomeric composition is determined using crystal-structure-referenced alignment and classification analysis [21], [22]. The percentage reported is computed from the total number of particles, N, comprising all PA7 and PA8 classes, where oligomeric composition is equal to the total number of PA7 or PA8 particles divided by N.en.d., not determined. Class-average image analyses of these pH conditions are not shown due to the low particle counts observed. The low particle counts are attributed to severe aggregation (as shown in Fig. 3A).