The Molecular Dissection of the Oncogenic Role of ETS1 in the Mesenchymal Subtypes of Head and Neck Squamous Cell Carcinoma [RNA-seq knock-down]

RNA-Sequencing Analysis of gene expression changes due to ablation of Ets1 in SCC25 Cells. Overall design: Global transcriptomic characterization of the effects of Ets1 knockdown in SCC25 Head and Neck Squamous Cell Carcinoma cells was performed via RNA-Seq Analysis. The knockdown of ETS1 was performed using transient siRNA based approach as well as stable cell lines were generated with targeted shRNA. As controls, we used SCC25 cells transduced with siRNAs and shRNAs with non-silencing control hairpin sequences that do not match any known mammalian genes and hence are not expected to not target any gene product.