Metatranscriptome of field-collected Spodoptera frugiperda larvae guts from Tucuman province (Argentina). Spodoptera frugiperda

The purpose of this study is to perform a comprehensive analysis which integrates Spodoptera frugiperda gene expression data with environmental information and taxa associated with S. frugiperda larvae guts. For this, total RNA extracted from field-collected S. frugiperda larvae guts, captured from different maize (Zea mays) fields and from Sorghum halepense in Tucumán province (Argentina), was submitted to sequence-independent amplification and high-throughput pyrosequencing. Due to the fact that total RNA was amplified using a modified sequence-independent amplification protocol and, for this, first-strand reverse transcription was initiated with a random octamer linked to a specific primer sequence, an unbiased random collection of transcripts was obtained for each sample.