The RNA binding proteins ZFP36L1 and ZFP36L2 modulate transcriptional and post-transcriptional genome wide effects of glucocorticoids

To investigate the genome-wide role of RNA binding proteins ZFP36L1 and ZFP36L2 in glucocorticoid mechanisms of action in bronchial epithelial cells Overall design: Primary BECs were depleted of ZFP36L1 and ZFP36L2 and stimulated with dexamethasone overnight. Frac-seq was performed to allow comparison of total mRNA to monosomal (80S) and polyribosomal (poly) bound mRNAs RNA-seq was performed for each fraction (total, 80S, poly) comparing the effects of knockdown and of dexamethasone (DEX)