Canine Polarized Macrophages Express Distinct Functional and Transcriptomic Profiles. Canine Polarized Macrophages Express Distinct Functional and Transcriptomic Profiles

in the present study we investigate polarized canine macrophages using transcriptome sequencing, a larger panel of flow cytometry markers, and antimicrobial functional assays. Transcriptome analysis of primary canine monocyte-derived macrophages revealed unique, previously unreported signatures for polarized M1 and M2 macrophages. Overall design: canine monocyte derived macrophages were differentiated into inflamtory or anti-inflmatory phenotypes then subjected to bulk RNA sequencing