Next Generation Sequencing Quantitative Analysis of TEAD Inhibitors in NCI-H226 cells

Purpose: The goal of this study is to understand the signaling pathway alteration in NCI-H226 cells treated with TEAD inhibitors. Methods: Mesothelioma cell line NCI-H226 was chosen to be treated with TEAD inhibitors at 1µM for 24 hours. Total RNA was isolated for the analysis. RNA samples were sent to Novogen for library construction, RNA sequencing and raw data process. Conclusions: Our study privides gene expression profiling evidence to validate TEAD inhibitors to block TEAD transcriptional activity in mesothelioma cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for NCI-H226 cells treated with vehicle control and TEAD inhibitor TM2, K975 and VT103.