Fosmid clones with transport-mediated genes imparting salt tolerance to E. coli, constructed from metagenomic DNA of brine waters of Sambhar Lake, India

Finding of transport mediated gene from extreme halophile environmentTo identify transport mediated gene(s) from extreme halophiles of Sambhar Lake in Rajasthan a fosmid library of ~50000 clones was prepared and direct sequencing of fosmid DNA (SC_1 and SC_2) on NGS platform (ION S5).