Human adipose tissue-derived mesenchymal progenitor cells induced toward adipogenic fates for 7 days and 2 subpopulations were separated and collected by percoll density gradient

The study aims to identify gene signature of 7-day adipogenic induced cells for the high density (non-differentiated) and low-density (adipocyte) cells. Overall design: Human adipose tissue derived mesenchymal progenitor cells were grown under adipogenic inducing condition for 7-cays and placed into a percoll density gradient. After centrifugation, cells were separated into two populations (high and low densities) and collected for bulk RNA sequencing