pattern of miR-22 knockout mouse BAT gene expression

To further understand different gene expression of miR-22 knockout mouse BAT and normal BAT, we have employed BAT samples microarray expression profiling as a discovery platform to identify different genes with miR-22 knockout mouse BAT and normal BAT.comparision with normal BAT,significantly upgene is 522 and downgene is 720 in knockout group. 3 control and 3 miR-22 knockout mice BAT samples were flow separated into trizol, the samples were taken to lab on ice, mRNA were extracted with trizol, good quality mRNA were choosen as microarrary analysis in capitalBio technology.