The ability of clonal bacterial populations to generate genomic and phenotypic heterogeneity is thought to be of great importance for many commensal and pathogenic bacteria. One common mechanism contr
Structural basis for broad DNA-specificity in integron recombination Descriptor: DNA (31-MER), DNA (34-MER), site-specific recombinase IntI4 Authors: MacDonald, D, Demarre, G, Bouvier, M, Mazel, D, Go
Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added t
Donor cells are II1 with pCIG1077 and pR6K::oriTP oriTw, and recipient cells are DH5α (IHF+) or CIG1 (isogenic IHF−) containing the indicated recipient plasmid with (+TrwA) or without (−TrwA) plasmid
