Cell death induction activity of MtNFP-sYFP2 truncated/mutated variants in Nicotiana leaves.

*-see [22], PM-plasma membrane, ER-endoplasmic reticulum.The designated constructs were expressed alone or co-expressed with MtLYK3-mCherry construct in Nicotiana leaves. Macroscopic symptoms of cell death were scored 48 hai: only infiltrations that resulted in confluent death of (nearly) the entire infiltrated region were scored and are presented as a fraction of total infiltrations performed. # - In the 3 remaining leaf regions, co-expression of MtNFP[S67A]-sYFP2 and MtLYK3-mCherry constructs resulted in increased staining with Evans blue in the entire infiltrated region.