Additional file 5: of Stability of gene expression by primary bronchial epithelial cells over increasing passage number

Figure S4. Un-normalized mRNA expression of innate immunity and immunomodulatory genes by primary BECs. Un-normalized mRNA Ct values for CXCL10 (A.), IFIH1 (B.), IL-33 (C.), and TSLP (D.) by BECs at P1 (n = 6 asthma donors, n = 6 healthy donors), P2 (n = 6 asthma donors, n = 6 healthy donors), P3 (n = 4 asthma donors, n = 6 healthy donors), P4 (n = 6 asthma donors, n = 6 healthy donors), and P5 (n = 6 asthma donors, n = 6 healthy donors) are presented as individual data points for each donor cell line. To compare expression of genes at P2-P5 to expression at P1, and to compare patterns of gene expression between asthmatic and healthy donors, ordinary two-way ANOVA with Dunnett’s multiple comparisons test was used for normally distributed data, and Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test was used for non-normally distributed data. (EMF 155 kb)