Impact of parental obesity on adipose progenitor programming

Parental obesity (PO) increases the risk of obesity and metabolic syndromes in offspring. To investigate how PO influences adipose progenitors, we generated offspring from lean (Ln-F1) or obese (Ob-F1) parents and isolated the stromal vascular fraction (SVF) from inguinal white adipose tissue (iWAT) depots at postnatal day 21 (P21). Bulk RNA sequencing of the SVF was performed to uncover the molecular mechanisms by which PO programs adipose progenitors. Overall design: To assess the effects of PO on the gene expression profile of adipose progenitors, RNA was isolated from the SVF of iWAT in P21 Ln-F1 and Ob-F1 male mice and subjected to bulk RNA sequencing.