Genomic integrity safeguards pluripotency in embryonic stem cells. Genomic integrity safeguards pluripotency in embryonic stem cells

Illumina BeadChip array analyses of mouse embryonic stem cell gene expression profiles in the presence of or upon knockdown of 4 candidate factors regulating genomic integrity -- Chek1, Ppm1g, Ppp2r1b and Bub1b. An ES complementation 'rescue' system was employed to measure the effects of knockdown (minus Dox). The 4 candidate factors are rescued to wildtype levels in the presence of Dox (plus Dox). A control rescue ES cell line, with a Luciferase-targeting shRNA, was also assessed. Overall design: Total of 30 samples; Chek1_R (+/-Dox), Ppm1g_R (+/-Dox), Ppp2r1b_R (+/-Dox), Bub1b_R (+/-Dox), Control_R (+/-Dox); all performed in triplicates.