BulkRNAseq - Neurotransmitters regulate distinct phases of multimodal human interneuron migration

We use cerebral organoid fusions and a novel migration tracking platform to understand the functional impact of neurotransmitter signaling on migrating human interneurons. Overall design: Seven unique groups: (I) GFP- cells – single dorsal organoids (II) GFP+ cells – single ventral organoids (III) GFP- cells – single ventral organoids (IV) GFP+ cells – dissected ventral regions of fusions (V) GFP- cells – dissected ventral regions of fusions (VI) GFP+ cells – dissected dorsal regions of fusions (VII) GFP- cells – dissected dorsal regions of fusions. In total, 32 single organoids and 34 fusions were used to obtain 52 samples for the RNA sequencing analysis.