Lipophilic fluorescent dye (DiD) transfer depends on TraA and cell motility.

Column headers list relevant properties of isogenic recipients. The same nonmotile (DK8601) donor was used in all mixtures and recipients were DW1414 (A+ traA+), DW1416 (A+ traA−) and DK8606 (A− traA+). The ability of the DiD dye (red fluorescence) to be transferred to GFP recipients was assessed in merged panels (100× objective). Here yellow color indicates DiD transfer to GFP recipients and representative cells are noted by arrows.