miRNA expression in oxygen-stable HIF1 transgenic mice. miRNA expression in oxygen-stable HIF1 transgenic mice

Oxygen-stable HIF1 was inducibly expressed in cardiomyocytes of double transgenic mice. Specifically, tetracycline-inducible HIF-PPN mice (Pro402, Pro564 and Asn803 of HIF1a were mutated to Ala residues to limit degradation, termed HIF-PPN) were crossed to a-MHC-tTA expressing mice. HIF-PPN expression was induced by removal of doxycycline for 3 days and compared to control hearts that remained on doxycycline (uninduced). Overall design: Two hearts per condition (3 days off dox and control- on dox) were pooled and hybridized on same array. Representative HIF induced hearts were selected based on significant increase in heart weight/body weight ratio compared to control hearts.