Strains, plasmids and oligonucleotides used in this work.

* Antibiotic-resistance phenotypes: Apr, ampicillin; Tcr, tetracycline; Emr, erythromycin. Antibiotic-resistance phenotypes and other features listed in parentheses are those expressed by F. psychrophilum but not by E. coli. Restriction sites are in bold type. ISce-I restriction site is underlined. Strains, plasmids and oligonucleotides used in this work.