Transcriptome of primary KP lung adenocarcinoma cells with Nupr1 knockout
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP532380
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资源简介：
The aim for this bulk mRNA sequencing project is to understand the age-specific impact of Nupr1 suppression in lung adenocarcinoma by CRISPR-Cas9 mediated gene knockout in vivo. Overall design: Lung adenocarcinomas were induced in aged (> 2 years old) vs young (12-16 weeks old) Kras LSL-G12D/+; Trp53 fl/fl; Rosa26-LSL-EGFP-Cas9 (KPCas9) mice. Mice were intubated with lentivirally delivered Cre recombinase, together with a control or two sgRNAs targeting murine Nupr1. Ten weeks after tumor induction, GFP positive tumor cells were sorted by FACS from both aged and young tumor-bearing mice and subjected to mRNA sequencing.
创建时间：
2024-09-16
