Leaf age dependent transcriptome analysis in response to abscisic acid

Since development is an important regulator in hormonal responses we wanted to investigate to what extent responses to the phytohormone abscisic acid (ABA) are controlled by old and young leaves within a single plant. Our RNAseq analysis indicate that ABA treatment triggers as set of common and leaf age specific responses in both old and young leaves. 4-5 week old Arabidopsis thaliana Col-0 plats were sprayed with either control (0.5% EtOH + 0.05% Tween20) or ABA (500µM, 100 mM stock in EtOH, 0.05% Tween20) treated and old leaves (true leave 7) and young leaves (true leave 12) were harvested 48h after treatment. The experiment was repeated 3 times and in total 12 samples were used for statistical analysis.