Genome wide localization of the NuRD complex in Ewing sarcoma cells [CHIP-seq]

Chromatin immunoprecipitation followed by DNA sequencing of 4 NuRD complex subunits as well as for the fusion transcription factor EWS-FLI1 and relevant histone marks in Ewing sarcoma cells were performed to characterize the binding sites of the chromatin remodeling complex in relation to the fusion oncoprotein. Overall design: Chromatin immunoprecipitation followed by DNA sequencing for FLI1, the NuRD subunits: CHD4, HDAC2, MTA2, and RBBP4, as well as for the histone marks H3K27ac, H3K27me3, H3K4me1 and H3K4me3 in Ewing sarcoma cells (SKNMC).