Bacillus subtilis produces high concentrations of amylase. The purpose of this study is to understand the genetic characteristics of this strain through whole gene analysis.
This strain, a derivative of Bacillus subtilis subsp. subtilis 168, is deficient in eight extracelluar proteases and used as a host for expression of heterologous proteins.
Bacillus was fermented from soybean and rice stalk, and vitamin K2 was produced by the strain. The yield of vitamin K2 was improved by optimizing the culture conditions