In situ detection of the eIF4F translation initiation complex in mammalian cells and tissues

The control exerted by the eukaryotic translation initiation complex eIF4F on gene expression has been increasingly demonstrated. The methods that are used to monitor the formation of the eIF4F complex are usually indirect and provide no information on its subcellular localization. This protocol describes a proximity ligation assay (PLA)-based procedure allowing the direct in situ visualization of the eIF4F complex, as well as its absolute quantification per cell using an adapted image analysis software. In this dataset, it comprises the supplemental files related to this article.