Functional validation of pathway inhibitors.
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Decidualized St-T1b were preincubated for 1 h with Y27632 (100 µM), NSC23766 (50 µM), Wortmannin (200 nM) (A) or PD98059 (50 µM), LY294002 (10 µM), SB202190 (10 µM) (B), followed by 5 min stimulation with PDGF-BB, TCM or IL-1β. Control cultures received Opti-MEM. Total or phosphorylated (p-) Akt, p38, ERK1/2 and MLC2 were detected by Western blot analysis.
创建时间：
2016-02-24
