Dual RNA-seq of Neisseria gonorrhoeae and human neutrophils

Deep sequencing of cDNA from Neisseria gonorrhoeae bacteria and human neutrophils, alone and after coincubation. Total RNA was isolated from Neisseria gonorrhoeae and adherent primary human neutrophils, alone and after co-culture over time. Bacteria were of strain FA1090 ?opa ("Opaless"; 130), FA1090 ?opa with H041 penA, penB, and mtrR resistance alleles (3x130), or H041. Bacteria were untreated or treated with experimentally determined sublethal concentrations of selected antibiotics 1 hour prior to and during co-culture. Ribosomal RNA was depleted and the remaining RNA was reverse-transcribed into a cDNA library for Illumina sequencing. Reads were mapped to the FA1090, H041 and/or human genomes.