INSECTICIDAL POLYPEPTIDES WHICH HAVE ONE SPECTRUM OF IMPROVED ACTIVITY and THEIR USES

Provided are compositions and methods for controlling pests. The methods involve transforming organisms with one sequence of nucleic acid that encodes an insecticidal protein. In particular， the sequences of nucleic acid are useful for preparing plants and microorganisms that possess insecticidal activity. Therefore， are provided by bacteria， plants， plant cells， plant tissues and seeds transformed. The compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences are useful for the construction of vectors of expression for its further processing in organisms of interest include plants and that probes for the isolation of other homologous genes (or partially homologous). The pesticidal proteins are useful for controlling， inhibiting the growth or take out populations of pests of lepidoptera， coleoptera， flies， fungi， hemipteran and nematodes and for producing compositions with insecticidal activity. Claim 1: One construction of DNA that comprising: to) one polynucleotide that encodes one polypeptide IPD103 which has insecticidal activity; and b) one or more polynucleotides selected from of: i) one polynucleotide that encodes one polypeptide PtIP-83 which has insecticidal activity; ii) one polynucleotide that encodes one polypeptide Cry1B which has insecticidal activity; iii) one polynucleotide that encodes one Cry1B variant polypeptide which has insecticidal activity; iv) one polynucleotide that encodes one polypeptide Cry1C which has insecticidal activity; v) one polynucleotide that encodes one polypeptide Cry1D which has insecticidal activity; and vi) One polynucleotide that encodes one polypeptide Cry1J which has insecticidal activity.