Diamond annotation
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p { margin-bottom: 0.25cm; direction: ltr; line-height: 120%; text-align: left; orphans: 2; widows: 2 } a:link { color: #0000ff } Annotation list for <i>Calanus finmarchicus</i> reference transcriptome using DIAMOND<sup>3</sup>. Contigs were aligned with DIAMOND??<sup>3</sup> on NR (2019-09-29), Swissprot and Trembl (2018-12) to retrieve corresponding best annotations. An annotation matrix was then generated by selecting the best hit for each database if: i) the percent of the query length covered by the alignment was higher than 60% ; ii) the percent of the subject length covered by the alignment was higher than 40%; iii) the percent of identity of the alignment was higher than 40%. File <b>diamond_annotation_206k.tsv </b>is the annotation list for <i>Calanus finmarchicus</i> reference transcriptome using DIAMOND<sup>3</sup> (36,274 contigs with an annotation in at least one database out of the 206,012). File <b>diamond_annotation_76k.tsv </b>is the annotation list for the 76,550 contigs expressed with more than 1 CPM in the RNA sequencing Bioproject PRJNA628886 using DIAMOND<sup>3</sup> (22,527 contigs with an annotation in at least one database out of the 76,550). <br> <sup>3</sup>DIAMOND: version v0.9.22, parameters: -f 6 qseqid qlen qcovhsp pident score evalue length sseqid slen stitle. <i>Ref: Buchfink, B., Xie, C. & Huson, D. H. Fast and sensitive protein alignment using DIAMOND. Nat. Methods 12, 59–60 (2015).</i> Related to bioproject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA628886
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figshare
创建时间:
2020-10-29



