Hyaluronidase activity of sTMEM2 (SEC)
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Size exclusion chromatography of 100 μg/ml fluorescein-labelled high-molecular-weight hyaluronan (HMW-HA, ~800 kDa, Carbosynth) or hyaluronan 22-mer (dp22, ~5 kDa, Iduron), and of 100 μg/ml fluorescein-labelled HMW-HA incubated overnight with 100 μg/ml transmembrane protein 2 ectodomain (sTMEM2) or sperm hyaluronidase (HAase, Sigma-Aldrich, H3884). The reaction buffer was 100 mM MES, pH 6.0, 1 mM CaCl<sub>2</sub>.
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figshare创建时间：
2023-01-30
