RNA sequencing data from the guts of Drosophila wildtype and CG3740/nazo double mutant 20-day-old males

Lipid dyshomeostasis has been implicated in a variety of diseases ranging from obesity to neurodegenerative disorders such as Neurodegeneration with Brain Iron Accumulation (NBIA). Here, we uncover the physiological role of Nazo, the Drosophilamelanogaster homolog of the NBIA-mutated protein – c19orf12, whose function has been elusive. Ablation of Drosophila c19orf12 homologs leads to dysregulation of multiple lipid metabolism genes. nazo mutants exhibit markedly reduced gut lipid droplet and whole-body triglyceride contents. Consequently, they are sensitive to starvation and oxidative stress. Nazo is required for maintaining normal levels of Perilipin-2, an inhibitor of the lipase – Brummer. Concurrent knockdown of Brummer or overexpression of Perilipin-2 rescues the nazo phenotype, suggesting that this defect, at least in part, may arise from diminished Perilipin-2 on lipid droplets leading to aberrant Brummer-mediated lipolysis. Our findings potentially provide novel insights into th..., The samples( guts) were dissected in ice-cold PBS and flash-frozen in liquid nitrogen followed by RNA extraction using a standard RNA extraction kit (Qiagen) before being processed. , , # Title: RNA Sequencing data from the guts of Drosophila wildtype and CG3740/nazo double mutant 20 day old males.

* Authors: Sreejith Perinthottathil, Rajnish Bharadwaj
* Date created: 2022-09-19
* Date modified: 2022-09-20

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* Dataset Title: Data for the article \"Nazo, the Drosophila homolog of the NBIA-mutated protein – c19orf12, is required for
triglyceride homeostasis\"

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* Dataset Contributors:

* Creators: Perinthottathil Sreejith, Sara Lolo, Kristen R. Patten, Maduka Gunasinghe, Neya More, Leo J.Pallanck, Rajnish Bharadwaj

* Date of Issue: 2022-09-30

* Publisher: University of Rochester

## Contact Information

* Name: Rajnish Bharadwaj

* Affiliations: Pathology and Lab Medicine, University of Rochester Medical Center

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* Alternative Contact: postdoctoral PI
* Name: Sreejith Perinthottathil
* Affiliations: Pathology and Lab Me...