Integrative Analysis of Lung Adenocarcinoma (Version 2)

We performed a comprehensive analysis of intratumor heterogeneity of somatic mutations in cancer driver genes, copy number aberrations and DNA methylation in 292 tumor samples from 84 lung adenocarcinoma (LUAD) patients from the Environment And Genetics in Lung cancer Etiology (EAGLE) study. SNVs of 37 established lung cancer driver genes were profiled by deep target sequencing in 180 tumor samples and 55 blood samples and 1 buccal sample from 56 subjects. Somatic copy number alterations were profiled using SNP arrays in 268 tumor samples from 80 subjects. DNA methylation levels were profiled with methylation arrays in 205 tumor samples and 74 normal tissue samples from 68 subjects.]]> Lung adenocarcinoma cases only.]]> Large population-based case-control study conducted in the Lombardy region of Italy between April 2002 and February 2005. All subjects were of Italian nationality between the ages of 35 and 79 years, official residents of the catchment area, and with no severe disease that could impede participation. Cases were newly diagnosed primary cancers, verified by tissue pathology. Lung tissue samples were snap-frozen in liquid nitrogen within 20 min of surgical resection. ]]>