Tcf-1 shapes the chromatin landscape of naive T cells. Mus musculus

We investigate the requirement of the transcription factor Tcf-1 for T cell specific accessible elements and gene expression in developing T cells. We examine the impact of Tcf-1 on creating chromatin accessibility related to T cell identity in an unrelated, differentiated cell-type. Overall design: Examination of chromatin accessibility in developing DP T cells from WT and Tcf-1 KO. Examination of chromatin accessibility, repressive histone modifications, and binding of Tcf-1 after ectopic expression in NIH3T3 fibroblasts.