CLIP (NOVA1 & AGO) and RNAseq (control & NOVA1KD) in human GBM cells

GBM cells derived from a patient were subjected to CLIP and RNAseq assays to for transcriptome analysis (CLIP and RNAseq). Overall design: GBM cells derived from a patinet were UV-irradiated and subjected to CLIP with anti-NOVA1 antibody (ab183024 from Abcam) and anti-AGO antibody (2A8: from Dr. Zissimos Mourelatos) and RNAseq (shControl vs shNOVA1KD1 & 2). mRNA abundance and translation efficiency profiles by deep sequencing using Illumina Novaseq. Detailed desription in accompanying paper.