Analysis of mRNA expression changes caused by mdt-15(tm2182) mutation at 15oC and 25 oC

To determine the role of MDT-15 in mRNA expression changes at different temperatures, we sequenced mRNAs obtained from day 1 adult Caenorhabditis elegans. Bristol N2 (wild-type) and mdt-15(tm2182) mutant animals that cultured at 25oC or 15oC were used. Measurement of mRNA levels in Bristol N2 (wild-type) and mdt-15(tm2182) animals cultured at 25oC or 15oC by deep sequencing (Macrogen, South Korea) in triplicate