LPS-induced cochlear inflammation and SPT-2101 treatment

[Methods] ABR DATA: obtained by ABR recording in a Tucker David Technology RZ6 Auditory Workstation using BioSigRZ software EVANS BLUE DATA: obtained by fluorimetry in a Glomax??? Luminometer and Microplate Reader (Promega) (620/680 nm excitation/emission wavelength). GENE EXPRESSION DATA: obtained by Real-Time PCR and analyzed by QuantStudio??? Real-Time PCR software 1.3. PCR array obtained using the PCR array RT?? Profiler??? Rat Innate & Adaptive Immune Responses (Qiagen, #PARN-052Z) and analyzed with Geneglobe (Qiagen). MRI DATA: images obtained with a horizontal 7.0-Tesla Bruker BioSpec?? system (Bruker Medical GmbH) running ParaVision 6.0.1 on a Linux environment. Data analysis was performed with Fiji software. OPTICAL IMAGING DATA: images obtained with a Zeiss AxioPhot microscope (Carl Zeiss). Data analysis was performed with Fiji software. FLUORESCENCE DATA: images obtained with an epifluorescence (Nikon 90i) and confocal laser-scanning (Zeiss LSM710) microscopes. Data analysis was performed with Fiji software. WB DATA: Immunoreactive bands revealed using the Clarity??? Western ECL Substrate (Bio-Rad), and images were captured with the ImageQuant LAS4000 mini digital camera using ImageQuant TL software 8.1 (GE Healthcare).