Supplementary material data for: Unstable environmental conditions constrain the fine-tune between opsin sensitivity and underwater light in an Amazon forest stream fish

Visual adaptations can stem from variations in amino acid composition, chromophore utilization, and differential opsin gene expression levels, enabling individuals to adjust their light sensitivity to environmental lighting conditions. In stable environments, adaptations often involve amino acid substitutions, whereas in unstable conditions, differential gene expression may be a more relevant mechanism. Amazon forest streams present diverse underwater lighting conditions and experience short-term water colour fluctuations. In these environments, it is less likely for genetic and amino acid sequences to undergo modifications that tailor opsin proteins to the prevailing lighting conditions, particularly in species having several copies of the same gene. The sailfin tetra, Crenuchus spilurus, inhabits black and clear water Amazon forest streams. The long wavelength sensitivity (LWS) is an important component for foraging and courtship. Here, we investigated LWS opsin genes in the sailfin t..., These are the nucleotide sequences used for the phylogenetic relationship among the genes found in our samples with those genes that have been previously described, and the data used to measure the variation in the underwater lighting condition in black and clear water Amazon streams., , # Data for Supplementary Material -

## Description of the data and file structure

These are the nucleotide sequences used for the phylogenetic relationship among the genes found in our samples with those genes that have been previously described, and the data (values in lux) used to measure the variation in the underwater lighting condition in black and clear water Amazon igarapés.

File = Phylogenetic_relationship_genes.fas

Lines 1 to 13 = NCBI sequence data (starting with the accession number) for each specific opsin gene and species that were used for the phylogenetic relationship among the genes found in our samples and those previously described.

Lines 14 to 19 = Genetic data obtained in the BW1 sample that was used for checking the phylogenetic relationship among the genes found in our samples with those opsin genes that were previously described and are available at NCBI.

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File name = Forest_stream_blue_light.csv

day = day of the year

time = time in which the data was...