In-vivo lysis of peptide-pulsed HLA-A2 cell targets by IGRP-specific CD8 T-cells.

Data represent the percentage of specific cytotoxicity against IGRP-peptide pulsed HLA-A2 EBV-LCL by IGRP-specific CD8 T-cells in two independent experiments. 4×106 IGRP-specific T cells were injected intrasplenically. One day later, mice received an i.v. injection containing of a 1∶1 mixture of specific peptide-pulsed CFSEhi target cells and control peptide-pulsed CFSElo target cells. At 20 hr, the ratio of CFSEhi and CFSElo cells in the spleens was analyzed by flow cytometry.*indicates a significant difference (p