Effect of KO of CCND1, ORAOV1, or miR548K in FaDu cancer cell lines or oral keratinocytes

To investigate the function of CCND1/CCND2/ORAOV1/miR548K in 11q13-amplified cancer cells or non cancerous keratinocytes, we established respective KO cells through CRISPR-Cas9. We performed gene expression profiling analysis using data obtained from RNA-seq upon target KO in either FaDu cells or TP53/CDKN2A k.o. oral keratinocytes Comparative gene expression profiling analysis of RNA-seq data for FaDu and TP53/CDKN2A KO oral keratinocytes KO derivatives (CCND1, CCND2, ORAOV1, miR548K, compared to 11q13-noncoding to control for copy number cuts).