Expression data of Gata4 endothelial cell-subtype specific knockout whole liver lysates

Liver sinusoidal endothelial cells (LSEC) constitute discontinuous, permeable microvessels, with a characteristic program of gene expression that differs significantly from continuous microvascular endothelial cells e.g. in the lung. Gata4 is described as master regulator of LSEC specification during liver development. Here, we sought to analyze the role of endothelial Gata4 in the adult liver. We used microarrays to analyse the program of gene expression in murine whole liver lysates with LSEC-specifig Gata4 deficiency. Murine whole liver lysates of Gata4 endothelial cell-subtype specific knockout (Gata4-LSEC-KO) and sibling control mice were isolated for RNA extraction and hybridization on Affymetrix microarrays.