Characterization of RNAs bound by THOC1 or LENG8

Given that LENG8 is associated with the THO complex and is required for mRNA processing, we went on to identify specific RNAs that bind LENG8 or THOC1. Therefore, we precipitated GFP-tagged THOC1, LENG8 or control GFP and sequenced their associated RNAs, using deep sequencing (RNA immune-precipitation followed by deep sequencing, RIP-Seq). We found that when LENG8 was used as bait, 13,328 transcripts from 5,796 genes, were enriched, whereas THOC1 was associated with 32,542 transcripts from 6,150 genes, and 5,179 transcripts from 2,979 genes were enriched in both RNA profiles of THOC1-GFP or LENG8-GFP immuno-precipitates from Hela cells