Comparison of different Y. pseudotuberculosis strains for survival inside macrophages as determined by CFU assay.
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BMDMs were infected with the indicated strains at an MOI of 10 for 20 min, followed by gentamicin treatment to eliminate extracellular bacteria. At 1 h and 23 h post infection, the infected BMDMs were lysed, and serial dilutions were plated to determine the survival of intracellular bacteria by CFU assay. Ratios of CFU at 23 h/1 h are shown, determined as [23 h post infection CFU/1 h post infection CFU]. Results shown are the means from three independent experiments with duplicate infection wells. Error bars show standard deviations. (A)***, P
创建时间:
2016-02-23



